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莠去津降解菌的筛选及其降解特性

冯瑞章1,2,杜永华1,2,魏琴1,2,周万海2*,何飞1,黄彭1,李莉1

1.(中国食品发酵工业研究院 中国工业微生物菌种保藏管理中心,北京 100015)

2.(日本 Combi株式会社,埼玉 338-0832)

Selection of atrazine-degrading strains and their degradation characteristics

Liu Yang1  Zhang Xin1  Chen Jianguo1  Liang Hanqiao1  Cheng Chi1*

Tetsuo ljichi2  Tatsuya Omori2

1 (China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China)

2 (Japan Combi Corporation, Saitama 3380832)

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摘要 

本文对屎肠球菌Enterococcus faecium EC-12的万古霉素耐药基因进行了筛查。通过PCR方法,以七种典型肠球菌万古霉素耐药基因(vanAvanBvanC1vanC2 /C3vanDvanEvanG)进行特异性扩增,结果均为阴性。PCR方法检测万古霉素耐药肠球菌简便、高效、准确,可用于肠球菌万古霉素耐药基因的快速筛查。

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关键词:  屎肠球菌  万古霉素  耐药基因  PCR     
Abstract: 

The atrazine-degrading strains were selectedusing high atrazine concentration solid culture and high performance liquid chromatography (HPLC), then the dynamics of growth and atrazine degradation rate were determined, and the effects of co-substrates and environmental condition ondegradation rate were also evaluated. The results showed that 10 strains had degradative capability for atrazinewith the degradation rate from 13.3% to 41.1%, and the maximum degradation rate was obtained with strain XQB-33. Among 10 strains, 4 strains (XQB-1, XQB-21, XQB-25, XQB-33) showed the higher atrazine degradation capacity (38.1%, 34.6%, 37.3% and 41.1%, respectively).Furthermore, 4 strains with higher degradative capacity reached logarithmic phase, and showed the highest atrazine degradation rate after 72h liquid medium culture. After addition of glucose, sodium citrate, sucrose and succinic acid sodium acetate as co-substrates, the degradation rate of 4 strains increased significantly, especially for 0.5% glucose, the degradation rate of XQB-1 and XQB-1 increased by 15.2% and 14.3%, respectively.The environmental factors can affect the degradation efficiency. The optimum reaction conditions for 4 strains degradation were inoculation amount 2.0%,initial concentration of atrazine 100-200 mg.L-1, initial pH value 5-6, culture temperature at 30℃, and volume at 75-100100mL/150mL.

Key words:  atrazine-degrading strains    dynamics of growth    degradation rate    co-substrates    environmental condition
                        
基金资助: 

四川省教育厅项目(13ZA0201);发酵资源与应用四川省高校重点实验室项目 (2010KFZ004,2011KFJ003);固态发酵资源利用四川省重点实验室项目(2015GTY006,2016GTY002)

通讯作者:  本文通讯作者,Email: wanhaizhou@126.com   
作者简介:  博士,副教授
引用本文:    
冯瑞章, 杜永华, 魏琴, 等. 莠去津降解菌的筛选及其降解特性[J]. 食品与发酵工业, 10.13995/j.cnki.11-1802/ts.015455.
FENG Rui-zhang et al.

Selection of atrazine-degrading strains and their degradation characteristics . Food and Fermentation Industries, 0, (): 1-.

链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.015455
[1] 陈志娜, 裴纪柳, 叶韬, 薛咏振, 詹志强, 李雅, 刘天. 泡菜源屎肠球菌Enterococcus faecium R2的环境胁迫耐受性及安全性评价[J]. 食品与发酵工业, 2019, 45(22): 32-38.
[2] 刘洋 , 张欣 , 陈建国 , 等. 粪肠球菌Enterococcus faecalis EC-12的万古霉素耐药基因检测 [J]. 食品与发酵工业, 2018, 44(3): 226-.
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