apparent enzyme activity of immobilized papain was taken as the index,the
effects of ultrasound frequency and power on the kinetics of immobilized papain
chemical reaction were investigated. It was found that the reaction of the immobilized papain
decompose casein is consistent with the first-order reaction rate formula.
Ultrasonic treatment can significantly reduce the apparent activation
energy.The ultrasonic power was 0.05W/cm2,and the ultrasonic
frequency was 28,40,50 and 135kHz,the apparent activation energy decreased
gradually,and the apparent activation energy was the lowest at 135kHz 0.25W/cm2,it
was38.23±0.53kJ/mol.When the ultrasonic frequency was
135kHz and the ultrasonic power is increased from 0.05W/cm2 to 0.45W/cm2,the apparent activation energy
was the lowest under the ultrasonic condition of 135kHz 0.25W/cm2,which
was 32.81.When the ultrasonic power continues to increase,the apparent
activation energy shows an upward trend.Under the ultrasound condition of 135kHz 0.25W/cm2,The
apparent activation energy was the lowest, which was 32.81±0.09kJ/mol,the
activation energy decreased by 52.48% .