Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of polysaccharides from Spirulina platensis
LUO Guanghong1,2, LEI Jie3, ZHANG Xifeng1,3*, JING Lina3, WANG Xinxin3, YANG Shenghui1,2, ZHANG Qingting3
1(Microalgae Technology Innovation Center of Gansu Province, Hexi University, Zhangye 734000, China) 2(Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, China) 3(School of Life Science and Engineering, Hexi University, Zhangye 734000, China)
Abstract: In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as an organic phase and sodium citrate (SC) as salt phase was used for partitioning of polysaccharides from Spirulina platensis (PSP). Results showed that the maximum extraction yield (EY) of PSP-D was (11.90 ± 0.12)% under the modified optimal conditions, DMC to crude extract volume ratio 0.6∶1.0, SC concentration 0.18 g/mL, 30 ℃, and pH 6.0. PSP-D had higher carbohydrate contents compared with the PSP-T, obtained from the TPP system with t-butanol and ammonium sulfate. PSP-D and PSP-T had different chemical compositions and molecular weights; however, their preliminary chemical structures remained unchanged. Moreover, PSP-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than PSP-T. Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.
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LUO Guanghong,LEI Jie,ZHANG Xifeng,et al. Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of polysaccharides from Spirulina platensis[J]. Food and Fermentation Industries, 2020, 46(17): 196-203.
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