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食品与发酵工业  2020, Vol. 46 Issue (17): 196-203    DOI: 10.13995/j.cnki.11-1802/ts.023788
  生产与科研应用 本期目录 | 过刊浏览 | 高级检索 |
碳酸二甲酯三相分配体系萃取分离螺旋藻多糖
罗光宏1,2, 雷婕3, 张喜峰1,3*, 敬丽娜3, 王鑫鑫3, 杨生辉1,2, 张青婷3
1(河西学院 甘肃省微藻技术创新中心,甘肃 张掖,734000)
2(甘肃省河西走廊特色资源利用重点实验室,甘肃 张掖,734000)
3(河西学院 生命科学与工程学院,甘肃 张掖,734000)
Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of polysaccharides from Spirulina platensis
LUO Guanghong1,2, LEI Jie3, ZHANG Xifeng1,3*, JING Lina3, WANG Xinxin3, YANG Shenghui1,2, ZHANG Qingting3
1(Microalgae Technology Innovation Center of Gansu Province, Hexi University, Zhangye 734000, China)
2(Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, China)
3(School of Life Science and Engineering, Hexi University, Zhangye 734000, China)
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摘要 以碳酸二甲酯为有机相,柠檬酸钠为盐相,采用三相分配体系(three-phase partitioning,TPP)对螺旋藻多糖(polysaccharides of Spirulina platensis,PSP)进行萃取分离。结果表明,在碳酸二甲酯与多糖粗提液体积比为0.6∶1.0、柠檬酸钠质量浓度为0.18 g/mL、温度为30 ℃、pH值为6.0的条件下,制备的螺旋藻多糖(PSP-D)的最大得率为(11.90±0.12)%。PSP-D比叔丁醇和硫酸铵形成TPP制备的多糖(PSP-T)具有更高的多糖含量,PSP-D和PSP-T具有不同的化学组成和分子质量,但其化学结构基本保持不变。PSP-D具有较强的自由基清除能力和总抗氧化能力,因此,碳酸二甲酯作为叔丁醇替代溶剂的TPP体系对天然生物分子的高效分配具有很大的潜力。
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罗光宏
雷婕
张喜峰
敬丽娜
王鑫鑫
杨生辉
张青婷
关键词:  螺旋藻多糖  三相分配法  碳酸二甲酯  萃取    
Abstract: In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as an organic phase and sodium citrate (SC) as salt phase was used for partitioning of polysaccharides from Spirulina platensis (PSP). Results showed that the maximum extraction yield (EY) of PSP-D was (11.90 ± 0.12)% under the modified optimal conditions, DMC to crude extract volume ratio 0.6∶1.0, SC concentration 0.18 g/mL, 30 ℃, and pH 6.0. PSP-D had higher carbohydrate contents compared with the PSP-T, obtained from the TPP system with t-butanol and ammonium sulfate. PSP-D and PSP-T had different chemical compositions and molecular weights; however, their preliminary chemical structures remained unchanged. Moreover, PSP-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than PSP-T. Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules.
Key words:  Spirulina platensis polysaccharides    three-phase partitioning    dimethyl carbonate    extraction
收稿日期:  2020-02-28      修回日期:  2020-05-07           出版日期:  2020-09-15      发布日期:  2020-10-14      期的出版日期:  2020-09-15
基金资助: 国家自然科学基金(11665011);甘肃省高校协同创新科技团队支持计划(2017C-17);甘肃省科技计划资助(18JR2JG001);甘肃省重点研发计划项目(18YF1WG087)
作者简介:  硕士,教授(张喜峰副教授为通讯作者,E-mail:curiouslysxsd@163.com)
引用本文:    
罗光宏,雷婕,张喜峰,等. 碳酸二甲酯三相分配体系萃取分离螺旋藻多糖[J]. 食品与发酵工业, 2020, 46(17): 196-203.
LUO Guanghong,LEI Jie,ZHANG Xifeng,et al. Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of polysaccharides from Spirulina platensis[J]. Food and Fermentation Industries, 2020, 46(17): 196-203.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.023788  或          http://sf1970.cnif.cn/CN/Y2020/V46/I17/196
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