Abstract: Yak milk is a kind of high-nutrition characteristic raw milk with excellent development potential. This paper used specific functional probiotics and conventional fermentation agents to develop high-quality yak probiotic fermented milk and studied the quality changes during fermentation and refrigeration. The effects of 3 strains of probiotics on the physical and chemical indexes (fermentation acidity, pH, water holding capacity and viscosity) of yak fermented milk were evaluated. The results showed that Lactobacillus salivarius AR809 had good fermentation performance and could be a potential probiotic starter. The effects of AR809 on the properties of yak fermented milk were further investigated. The addition of AR809 better controlled the post-acidification during the refrigeration stage and improved the water holding capacity of fermented milk. AR809 significantly alleviated the degradation level of yak milk protein, reaching 5.92%, which was 1.78 times that of the control group. Texture analysis indicated that adding AR809 could significantly increase the viscosity of fermented milk and enhanced the rheological properties of fermented milk. Sensory evaluation results confirmed that AR809 significantly promoted the palatability, tissue state, and adhesion of yak fermented milk. L. salivarius AR809 had good anti-inflammatory activity and could effectively alleviate chronic pharyngeal inflammation caused by Staphylococcus aureus. Using AR809 to prepare yak probiotic fermented milk, in addition to expanding the function of fermented milk, it can also effectively polish up the quality of fermented milk.
LIN X N, XIA Y J, WANG G Q, et al. Lactic acid bacteria with antioxidant activities alleviating oxidized oil induced hepatic injury in mice[J]. Frontiers in Microbiology, 2018, 9(11):1-10.
[5]
LIN X N, XIA Y J, WANG G Q, et al. Lactobacillus plantarum AR501 alleviates the oxidative stress of D-galactose-induced aging mice liver by upregulation of nrf2-mediated antioxidant enzyme expression[J]. Journal of Food Science, 2018, 83(7):1 990-1 998.
[6]
JIA G C, CHE N, XIA Y J, et al. Adhesion to pharyngeal epithelium and modulation of immune response: Lactobacillus salivarius AR809, a potential probiotic strain isolated from the human oral cavity[J]. Journal of Dairy Science, 2019, 102(8):6 738-6 749.
MANE A, CIOCIA F, BECK T K, et al. Proteolysis in Danish blue cheese during ripening[J]. International Dairy Journal, 2019, 97:191-200.
[11]
SAHINGIL D, GOKCE Y, YUCEER M, et al. Optimization of proteolysis and angiotensin converting enzyme inhibition activity in a model cheese using response surface methodology[J]. LWT - Food Science and Technology, 2019, 99:525-532.
[12]
LIU L, QU X W, XIA Q N, et al. Effect of Lactobacillus rhamnosus on the antioxidant activity of Cheddar cheese during ripening and under simulated gastrointestinal digestion[J]. LWT - Food Science and Technology, 2018, 95:99-106.
蒋萌蒙,王娟,刘欣,等. Lactobacillus casei Zhang在无糖枸杞酸奶中的应用[J]. 黑龙江畜牧兽医, 2017(5):131-134.
[19]
MELETHARAYIL G H, METZGER L E, HASMUKH A P. Influence of hydrodynamic cavitation on the rheological properties and microstructure of formulated Greek-style yogurts[J]. Journal of Dairy Science, 2016, 99(11): 8 537-8 548.
[20]
CRISPÍN-ISIDRO G, LOBATO-CALLEROS C, ESPINOSA-ANDREWS H,et al. Effect of inulin and agave fructans addition on the rheological, microstructural and sensory properties of reduced-fat stirred yogurt[J]. LWT - Food Science and Technology, 2015, 62(1): 438-444.
[21]
SAYAKA I, MAKOTO N, TAKAHIRO F, et al. Swallowing profiles of food polysaccharide gels in relation to bolus rheology[J]. Food Hydrocolloids, 2011, 25(5): 1 016-1 024.
[22]
KIP P, MEYER D, JELLEMA R H. Inulins improve sensoric and textural properties of low-fat yoghurts[J]. International Dairy Journal, 2006, 16(9):1 098-1 103.
[23]
TAKUNRAT T, MARUJ L, WANWIMOL K. Astaxanthin encapsulated in beads using ultrasonic atomizer and application in yogurt as evaluated by consumer sensory profile[J]. LWT - Food Science and Technology, 2015, 62(1): 431-437.