采用包埋法将乳酸菌UV3-9固定化处理后,对该固定化乳酸菌小球进行了发酵生产共轭亚油酸(CLA)的研究。结果显示,固定化小球的最佳直径为2～3 mm,固定化小球在氯化钙和硼酸混合液中需浸泡过夜;固定后的小球发酵产CLA最佳条件为:发酵温度37℃,初始pH 6.5,发酵时间28 h,底物LA添加量为0.3%,CLA最大产量达到92.061μg/mL。该固定化乳酸菌小球已完成了7批次的共轭亚油酸发酵,其产量均在80.476μg/mL以上。而且固定化UV3-9的CLA发酵产量是游离UV3-9的1.28倍。

This paper focused on the fermentation of conjugated linoleic acid(CLA) using immobilized lactic acid bacteria through embedding method.The results showed that the best diameter of immobilized ball was 2~3 mm.Furthermore,immobilized ball should be immersed in the mixture of calcium chloride and boric acid overnight.The best ferment conditions of immobilized ball were as follows: fermentation temperature of 37 ℃,initial pH of 6.5,fermentation time of 28 h,the substrate adding amount of 0.3 %,and maximum yield of CLA reached 92.061 μg/mL.7 batches of conjugated linoleic acid fermentation have been carried out using the immobilized lactic acid bacteria ball,and their outputs were higher than 80.476 μg/mL.And fermentation production of CLA using immobilized UV3-9 was 1.28 times of which using free UV3-9.