为探讨脂质氧化对蛋白质氧化的影响,用不同浓度2,2-偶氮二(2-甲基丙基咪)二盐酸盐[2,2'-azobis(2-amidinopropane) dihydrochloride,AAPH]热解产生的脂质初级代谢产物过氧自由基处理兔肉肌原纤维蛋白(myofibril protein,MP)。结果表明,随着AAPH浓度的增加,兔肉MP羰基含量、表面疏水性、亮度值和黄度值显著增加(P<0.05),而MP总巯基、游离氨、溶解度、内源性荧光强度和Zeta电位绝对值都呈显著降低趋势(P<0.05)。拉曼光谱结果显示,MP的α-螺旋含量下降,无规则卷曲含量上升,进一步证实蛋白质二级结构发生改变。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gelelectrophoresis, SDS-PAGE)结果表明,AAPH会导致MP发生交联和聚集,形成分子质量较大的聚集物。因此,过氧自由基会通过促进兔肉肌原纤维蛋白氧化,影响MP的理化性质,研究可为通过调控脂质氧化来控制兔肉加工过程中的蛋白氧化提供一定的理论依据。
In order to study the effect of lipid oxidation on protein oxidation, the primary lipid metabolite peroxyl radicals produced by 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) was used to treat rabbit myofibrillar protein (MP). The results showed that with the increase of AAPH concentration, the carbonyl content, surface hydrophobicity, brightness and yellowness of rabbit meat MP increased significantly (P<0.05). However, the total sulfhydryl group, free ammonia, solubility, endogenous fluorescence intensity and the absolute value of Zeta potential of rabbit meat MP showed a significant decreasing trend (P<0.05). Moreover, the results of Raman spectroscopy showed that the α-helix content of MP decreased and the content of irregular coils increased, which further confirmed that the protein secondary structure had changed. Besides, sodium dodecyl sulfate-polyacrylamide gelelectrophoresis (SDS-PAGE)results showed that the pyrolysis products of AAPH could cause cross-linking and aggregation of MP, forming aggregates with larger molecular weight. As a result, peroxyl radicals can affect the physicochemical properties of MP by promoting the oxidation of rabbit meat myofibril protein, which provides a theoretical basis for controlling the oxidation of protein during the processing of rabbit meat by regulating lipid oxidation.
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