食品与发酵工业 >

2023 , Vol. 49 >Issue 7: 153 - 158

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032302

研究报告

一种CAS-镉(Ⅱ)平板覆盖法鉴别微生物分泌铁载体对镉的螯合能力

  • 关文浩 ,
  • 王奥巍 ,
  • 房志家 ,
  • 孙力军 ,
  • 刘颖 ,
  • 廖建萌
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  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东 湛江,524088)
    2(海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁 大连,116034)
    3(湛江市食品药品检验所,广东 湛江,524088)
硕士研究生(房志家副教授为通信作者,E-mail:fzj4437549@163.com)

收稿日期: 2022-05-11

  修回日期: 2022-05-28

  网络出版日期: 2023-04-28

基金资助

国家自然科学基金面上项目(32172215);国家自然科学基金青年项目(31701706);广东省自然科学基金面上项目(2019A1515010809,2021A1515012443)

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The Cd chelating capability of siderophores secreted by microorganisms was identified via a CAS-Cd plate covering

  • GUAN Wenhao ,
  • WANG Aowei ,
  • FANG Zhijia ,
  • SUN Lijun ,
  • LIU Ying ,
  • LIAO Jianmeng
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)
    3(Zhanjiang Institute of Food and Drug Control, Zhanjiang 524088, China)

Received date: 2022-05-11

  Revised date: 2022-05-28

  Online published: 2023-04-28

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摘要

为了更加直观和稳定地鉴别微生物分泌铁载体螯合镉的能力,采用铬天青S(chrome azurol S,CAS)-铁(Ⅲ)平板培养法确认菌株洋葱伯克霍尔德菌具有产铁载体能力,并在CAS-铁(Ⅲ)检测液的工作原理上改良得CAS-镉(Ⅱ)检测液。在CAS-镉(Ⅱ)检测液基础上分别采用菌上清液检测法、平板法和平板覆盖法尝试鉴别该菌分泌的铁载体是否具备螯合镉的能力。结果显示洋葱伯克霍尔德菌具有产铁载体的能力,液体检测法存在干扰因素,平板法则无法检测和培养细菌,唯独平板覆盖法能确保微生物生长,并在处理12 h后的菌落周围观察到明显的镉螯合褪色圈,说明通过CAS-镉(Ⅱ)平板覆盖法能够清晰稳定地鉴别微生物螯合镉的能力。该研究成果佐证了微生物分泌的铁载体对镉的螯合作用,为筛选螯合重金属的微生物提供了新方法和思路。

关键词： 镉; 洋葱伯克霍尔德菌; 铁载体; CAS-镉(Ⅱ)检测液

本文引用格式

关文浩 , 王奥巍 , 房志家 , 孙力军 , 刘颖 , 廖建萌 . 一种CAS-镉(Ⅱ)平板覆盖法鉴别微生物分泌铁载体对镉的螯合能力[J]. 食品与发酵工业, 2023 , 49(7) : 153 -158 . DOI: 10.13995/j.cnki.11-1802/ts.032302

Abstract

In order to more intuitively and stably identify the ability of microorganisms to secrete siderophores for chelating cadmium, CAS-Fe (Ⅲ) plate culture method was used to confirm that Burkholderia cepacia had the ability to secrete siderophores, and CAS-Cd (Ⅱ) detection solution was modified based on the working principle of CAS-Fe (Ⅲ) detection solution. On the basis of CAS-Cd (Ⅱ) detection solution, bacterial supernatant detection method, plate method and plate covering method were used to identify whether the siderophore secreted by the bacterium had the ability of chelating cadmium. The results showed that B. cepacia had the ability to secrete siderophore. The liquid detection method had interference factors, and the plate method could not detect or culture bacteria, but the plate covering method could ensure the growth of microorganisms, and obvious fading circles due to cadmium chelation were observed around the bacterial colonies after 12 h treatment. It was suggested that the CAS-Cd (Ⅱ) plate covering method could clearly and stably identify the ability of chelating cadmium in microbes. This study verified the effect of siderophores secreted by microorganisms on chelating cadmium, providing a new method and idea for screening microorganisms to chelate heavy metals.

Key words： cadmium; Burkholderia cepacian; siderophore; CAS-Cd (Ⅱ) detection solution

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