食品与发酵工业 >

2023 , Vol. 49 >Issue 9: 340 - 347

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032374

综述与专题评论

基于噬菌体的食源性致病菌检测方法研究进展

  • 孙新城 ,
  • 许素月 ,
  • 李侠颖 ,
  • 李爽 ,
  • 周杰 ,
  • 杨德良 ,
  • 胡金强 ,
  • 张晓根
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  • 1(郑州轻工业大学 食品与生物工程学院,河南 郑州,450001)
    2(河南省冷链食品质量安全与控制重点实验室,河南 郑州,450001)
    3(食品生产与安全河南省协同创新中心,河南 郑州,450001)
    4(河南德惠源生物技术有限公司,河南 郑州,450001)
博士,副教授(张晓根教授为通信作者,E-mail:mzzxg2009@126.com)

收稿日期: 2022-05-18

  修回日期: 2022-07-06

  网络出版日期: 2023-06-05

基金资助

河南省重大公益项目(201300110100);河南省人社厅留学回国人员择优支持项目(2020039);郑州轻工业大学校内重大培育项目(2020ZDPY0102,2021ZDPY0202) ;河南省科技攻关项目(222102520041)

收起

Advances in bacteriophage-based detection of foodborne pathogens

  • SUN Xincheng ,
  • XU Suyue ,
  • LI Xiaying ,
  • LI Shuang ,
  • ZHOU Jie ,
  • YANG Deliang ,
  • HU Jinqiang ,
  • ZHANG Xiaogen
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  • 1(College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China)
    2(Henan Provincial Key Laboratory of Cold Chain Food Quality Safety and Control, Zhengzhou 450001, China)
    3(Food Production and Safety Henan Collaborative Innovation Center, Zhengzhou 450001, China)
    4(Henan Dehuiyuan Biotechnology Co. Ltd., Zhengzhou 450001, China)

Received date: 2022-05-18

  Revised date: 2022-07-06

  Online published: 2023-06-05

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摘要

全球每年有几百万人因食源性致病菌感染引发疾病,亟需开发快速且专一的食源性致病菌检测方法以保障食品安全。噬菌体具有反应效率高、特异性强以及容易制取等众多优势,被广泛用于食源性致病菌检测。文章首先介绍噬菌体并且阐述食源性致病菌检测现状,接着分析噬菌体与微生物学、免疫学、分子生物学、光学以及生物传感器等技术联合检测食源性致病菌的原理,重点总结了噬菌体联合这些技术的检测方法在实际应用中的最新进展,以期为噬菌体在食源性致病菌检测中的应用提供参考。

关键词： 噬菌体; 食源性致病菌; 分子生物学检测; 免疫学检测; 生物传感器

本文引用格式

孙新城 , 许素月 , 李侠颖 , 李爽 , 周杰 , 杨德良 , 胡金强 , 张晓根 . 基于噬菌体的食源性致病菌检测方法研究进展[J]. 食品与发酵工业, 2023 , 49(9) : 340 -347 . DOI: 10.13995/j.cnki.11-1802/ts.032374

Abstract

Millions of people worldwide are infected by foodborne pathogens every year. It is urgent to develop fast and specific detection methods for foodborne pathogens to ensure food safety. Phages have many advantages such as high reaction efficiency, strong specificity and easy preparation, so they are widely used for detection of foodborne pathogens. Article first introduces the phage and expounds the current situation of detection of foodborne pathogens, and then analyzes the principle of phage combined with microbiology, immunology, molecular biology, optics and biosensor technology for detection of foodborne pathogens, and emphatically summarizes the latest progress of detection methods of phage combined with these technologies in practical application, so as to provide reference for the application of phage in detection of foodborne pathogens.

Key words： phage; foodborne pathogens; molecular biological detection; immunological detection; biosensor

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