食品与发酵工业 >

2025 , Vol. 51 >Issue 13: 234 - 242

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040739

研究报告

牛骨源血管紧张素转换酶抑制肽的鉴定与表征

  • 杨泽瑶 ,
  • 许静 ,
  • 王江雪 ,
  • 王子夜 ,
  • 席凡伟 ,
  • 曹松敏
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  • (宁夏大学 食品科学与工程学院,宁夏 银川,750021)
第一作者: 硕士研究生(曹松敏副教授为通信作者,E-mail:songmin_cao@nxu.edu.cn)

收稿日期: 2024-08-13

  修回日期: 2024-10-17

  网络出版日期: 2025-08-04

基金资助

宁夏回族自治区教育厅高等学校科学研究项目(NYG-2024-054);宁夏重点研发项目(引才专项)(2022BSB03063)

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Identification and characterization of angiotensin-converting enzyme inhibitory peptide from bovine bone

  • YANG Zeyao ,
  • XU Jing ,
  • WANG Jiangxue ,
  • WANG Ziye ,
  • XI Fanwei ,
  • CAO Songmin
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  • (School of Food Science and Engineering, Ningxia University, Yinchuan 750021, China)

Received date: 2024-08-13

  Revised date: 2024-10-17

  Online published: 2025-08-04

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摘要

畜骨作为肉品工业的主要加工副产物,一直未得到充分利用,附加值较低。该研究以牛骨为原料,利用可控酶解技术制备了具备高血管紧张素转换酶(angiotensin-converting enzyme, ACE)抑制活性的牛骨水解产物,然后通过多色谱柱串联及Nano-LC-ESI-MS/MS技术对其中的高活性ACE抑制肽进行了分离纯化及鉴定,最终鉴定得到具备高ACE抑制活性的牛骨肽(Gly-Phe-Ser-Gly-Phe-Arg,GPSGPR)(IC50=382.16 μmol/L);分子模拟对接结果显示GPSGPR中的氨基酸残基主要通过与ACE的3个活性口袋中活性位点形成稳定的氢键发挥ACE的抑制作用;大鼠血清酶抗实验结果表明,GPSGPR在大鼠血浆中具有较高的酶抗性,体外培养6 h后仍能保持50.01%的完整性。该研究结果表明牛骨在ACE抑制肽的制备方面具有较高的潜力,为畜产品加工副产物的高值化利用提供了一定的参考。

关键词： 牛骨; 高血管紧张素转换酶; 多肽; 分子对接; GPSGPR

本文引用格式

杨泽瑶 , 许静 , 王江雪 , 王子夜 , 席凡伟 , 曹松敏 . 牛骨源血管紧张素转换酶抑制肽的鉴定与表征[J]. 食品与发酵工业, 2025 , 51(13) : 234 -242 . DOI: 10.13995/j.cnki.11-1802/ts.040739

Abstract

Animal bones, as the main by-product of the meat industry, have not been fully utilized and possess low added value.In this study, bovine bone was used as the raw material to prepare a bovine bone hydrolysate with high angiotensin-converting enzyme (ACE) inhibitory activity using controllable enzymatic hydrolysis technology.The highly active ACE inhibitory peptide was then isolated, purified, and identified using multi-column tandem chromatography and Nano-LC-ESI-MS/MS technology.Finally, the bovine bone peptide (Gly-Phe-Ser-Gly-Phe-Arg,GPSGPR) (IC50=382.16 μmol/L) with high ACE inhibitory activity was identified.Molecular simulation docking results showed that the amino acid residues in GPSGPR mainly exert the inhibitory effect on ACE by forming stable hydrogen bonds with the active sites in the three active pockets of ACE.The results of rat serum enzyme resistance experiments demonstrated that GPSGPR exhibits high enzyme resistance in rat plasma, maintaining 50.01% integrity after 6 hours of in vitro cultivation.The results of this study indicate that bovine bone has high potential in the preparation of ACE inhibitory peptides, offering a valuable reference for the high-value utilization of by-products in animal product processing.

Key words： bovine bone; high angiotensin-converting enzyme (ACE); peptides; molecular docking; GPSGPR

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