研究报告

食源唾液酸糖肽糖基化修饰对病原微生物和老人肠道菌群的影响

  • 朱娇 ,
  • 吴剑荣 ,
  • 牛文璇 ,
  • 范群艳 ,
  • 柳训才
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  • 1(江南大学 生物工程学院,糖化学与生物技术教育部重点实验室,江苏 无锡,214122)
    2(厦门市燕之屋丝浓食品有限公司燕窝研究院,福建 厦门,361000)
第一作者:硕士研究生(吴剑荣副教授为通信作者,E-mail:kinowu@jiangnan.edu.cn)

收稿日期: 2025-01-08

  修回日期: 2025-04-04

  网络出版日期: 2025-12-15

基金资助

国家重点研发计划项目(2023YFA0914303)

Impact of dietary sialoglycopeptides glycosylation on pathogenic microorganisms and gut microbiota in elderly individuals

  • ZHU Jiao ,
  • WU Jianrong ,
  • NIU Wenxuan ,
  • FAN Qunyan ,
  • LIU Xuncai
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  • 1(School of Biotechnology, Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China)
    2(Bird’s Nest Institute, Xiamen Yan Palace Seelong Food Co.Ltd., Xiamen 361000, China)

Received date: 2025-01-08

  Revised date: 2025-04-04

  Online published: 2025-12-15

摘要

蛋白糖基化影响着糖蛋白的结构特性和生物学功能。可食用燕窝(edible bird’s nest,EBN)和酪蛋白糖巨肽是高度唾液酸化的糖蛋白和糖肽,前者含有N-聚糖和O-聚糖修饰,而后者仅含有O-聚糖。该研究比较了EBN糖肽和酪蛋白糖巨肽的生物功能,包括对白色念珠菌、幽门螺杆菌、禽流感病毒(avian influenza virus,AIV)等病原体的抑制作用,以及其对老年人肠道菌群结构的调节作用。对照组为去唾液酸的EBN糖肽、N-乙酰神经氨酸和3′-唾液酸乳糖。基于DNA测序仪的荧光糖电泳分析显示,EBN糖肽中存在具有唾液酸化的多天线N-聚糖和平分型N-乙酰氨基葡萄糖(N-Acetylglucosamine, GlcNAc)糖链。结果表明,相较于酪蛋白糖巨肽,EBN糖肽对白色念珠菌和幽门螺杆菌有显著抑制作用,去除其末端唾液酸可显著降低这种抑制作用(P<0.05)。同时发现,EBN糖肽可以有效抑制AIV对犬肾细胞(Madin-Darby Canine Kidney, MDCK)的黏附。粪菌体外厌氧发酵结果表明,EBN糖肽组对老年人肠道菌群有良好的益生特性。综上,糖蛋白的唾液酸化结构形式影响其生物活性,与单一O-糖基化结构的酪蛋白糖巨肽相比,富含N-聚糖和O-聚糖的EBN糖肽具有更优良的抑制病原微生物和调节老人肠道菌群的功能。

本文引用格式

朱娇 , 吴剑荣 , 牛文璇 , 范群艳 , 柳训才 . 食源唾液酸糖肽糖基化修饰对病原微生物和老人肠道菌群的影响[J]. 食品与发酵工业, 2025 , 51(22) : 89 -100 . DOI: 10.13995/j.cnki.11-1802/ts.042076

Abstract

Protein glycosylation modulates the structural properties and biological functions of glycoproteins.Edible bird’s nest (EBN) and casein glycomacropeptide (CGMP) are both highly sialylated glycoprotein and glycopeptide.EBN is characterized by the presence of both N-glycan and O-glycan modifications, whereas CGMP contains exclusively O-glycans.This study compared the biological functions of EBN glycopeptides and CGMP, including their inhibitory effects on pathogens such as Candida albicans, Helicobacter pylori, and avian influenza virus (AIV), as well as their modulatory effects on the structure of the intestinal flora in elderly individuals.The controls used in this study include desialylated EBN glycopeptides, N-acetylneuraminic acid, and 3′-sialyllactose.DNA sequencer-based fluorescent glycan electrophoretic analysis revealed that EBN contained multiantennary N-glycans with sialylation and bisecting N-acetylglucosamine glycan chains.Results indicated that EBN glycopeptides could significantly inhibit C.albicans and H.pylori compared to CGMP.This inhibitory activity was substantially diminished when the terminal sialic acid residues of the EBN glycopeptides were removed (P<0.05).It was also found that EBN glycopeptides could effectively inhibit the adhesion of AIV to MDCK cells.In vitro fecal fermentation using fecal samples from elderly individuals demonstrated that EBN glycopeptides exhibited remarkable probiotic effects on the gut microbiota.In conclusion, the sialylation structure of glycoproteins played a crucial role in determining their biological activities.EBN glycopeptides, which were rich in both sialylated N-glycans and O-glycans, demonstrated a robust ability to inhibit pathogenic microorganisms and exhibited superior probiotic properties compared to CGMP that contained only O-glycosylation.

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