In this study,anthocyanins were extracted from wild red raspberries with 0.5% TFA in ethanol solution and were subjected to quantitative analysis.The total anthocyanin content of red raspberry crude extract,which was determined by pH differential method,was(7.548±0.03) mg/g of freeze-dried powder.Furthermore,the extracts were purified on a AB-8 macroporous resin column,the optimum elution reagent was 70% ethanol with TFA(pH 1),elution of ethanol was 6 times column volume at flow rate either on adsorption or desorption of 2 mL/min.Nine major anthocyanins of red raspberry were isolated.All of the peaks are identified by low-resolution electro spray mass spectrometry.HPLC-ESI/MS analysis results showed that the component of anthocyanins of the wild red raspberry fruit are cyanidin-3,5-diglucoside,cyanidin-3-digalactoside,cyanidin-3-glucoside,cyanidin-3-rutinoside,cyanidin,cyanidin-3-arabinoside,cyaniding-3-(6-O-acetyl)-glucoside pelargonidin-3-glucoside and peonidin-3-glucoside.