Alkaline extraction method was used to extract protein from Cordyceps militaris.The protein yield was designed as the target index.Based on single-factor experiment,and the principle of Box-Behnken central composite design,response surface analysis(RSA) was applied to optimize the extraction conditions of protein from Cordyceps militaris.At the extracting temperature of 60℃,the optimum extraction conditions were: extrcating time 128 min,solid-liquid ratio 1∶ 16.2,NaOH solution concentration 1.4 mol/L.Under the above conditions,the highest protein yield of estimated and actual values were 88.19mg/g and 88.92%,respectively.