Established a specific,sensitive and stable detection method for pathogenic genes of Vibrio parahaemolyticus(VP).Methods The detection specificity,sensitivity and reproducibility of fluorescent PCR method established for the detection of pathogenic genes tdh,trh,and tlh of Vibrio parahaemolyticus were checked to verify the effectiveness of the method.The method was successfully applied to detect 6 standard strains and isolates of Vibrio parahaemolyticus without cross-reaction with other Vibrio and non-Vibrio strains.The detectable limits for three pathogenic genes were 6~43 CFU/mL for tlh,97~1 700 CFU/mL for tdh,1 100~4 000 CFU/mL for trh,respectively.The variation coefficient inside each of 20 repeating groups for these three genes were 0.96%~1.50%.The variation coefficient between groups was 2.70%~4.10%.The method is simple,specific,highly sensitive,and is capable of accurate,rapid,sensitive detection of Vibrio parahaemolyticus in aquatic products.