The effect of Lacticaseibacillus paracasei CCFM1349 on the prevention and regeneration of hair loss was investigated by establishing a C57BL/6 mice hair loss model.In the animal experiment, mice were administered L.paracasei CCFM1349 for 21 days, observing its effects on hair growth and the number of hair follicles in the skin tissue of alopecia mice.The expression levels of proteins related to the Wnt/β-catenin pathway and apoptosis pathway in the back skin of mice were determined using quantitative polymerase chain reaction, while ELISA was used to detect the levels of hair growth factor and hair growth inhibitory factor in mice skin.Results demonstrated that L.paracasei CCFM1349 significantly promoted hair growth in alopecia mice, with a 76.64% coverage rate observed at day 14.At the molecular level, after intervention with L.paracasei CCFM1349, mRNA expression levels for Wnt5a, Wnt10b, and LEF1 in the Wnt/β-catenin pathway were upregulated by factors of 2.09-fold, 4.67-fold, and 5.53-fold respectively.Furthermore, mRNA expression levels for anti-apoptosis factor Bcl2 were significantly upregulated by a factor of 3.51-fold while a factor of 16.69% downregulated mRNA expression levels for proapoptotic factor BAX.Simultaneously, there was a significant increase of VEGF (10.60%) and IGF-1(19.65%), both being important hair growth factors found within mice skin.The content of TGF-β1 was significantly decreased by 12.70%.Results indicated that L.paracasei CCFM1349 promote hair growth in mice by regulating the Wnt/β-catenin pathway, inhibiting apoptosis and promoting the expression of growth factors.
ZHANG Tongtong
,
TANG Xin
,
MAO Bingyong
,
ZHANG Qiuxiang
,
ZHAO Jianxin
,
CHEN Wei
,
CUI Shumao
. Effects of Lacticaseibacillus paracasei CCFM1349 on hair growth in mice[J]. Food and Fermentation Industries, 2025
, 51(6)
: 272
-279
.
DOI: 10.13995/j.cnki.11-1802/ts.038900
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