Food and Fermentation Industries >

2025 , Vol. 51 >Issue 20: 53 - 60

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042259

Screening of microbial strains with strong tyrosine conversion ability and its application in broad bean paste

  • GU Nianwen ,
  • CHEN Huating ,
  • LIU Chunfeng ,
  • NIU Chengtuo ,
  • LI Qi
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  • 1(Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2025-01-25

  Revised date: 2025-03-25

  Online published: 2025-10-27

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Abstract

Tyrosine is the main component of white spots in broad bean paste and soybean paste.Reducing the tyrosine content in mash can significantly reduce the probability of white spot formation.This study aimed to obtain microbial strains with strong tyrosine conversion ability and to apply in broad bean paste fermentation.Four microbial strains with potential tyrosine conversion ability were screened from broad bean paste mash and Baijiu mash and their salinity tolerance ability was tested.Bacillus subtilis R1 with relatively good salt tolerance was used as starting strain.Atmospheric pressure room temperature plasma technique was used for mutagenesis.After two-round screening, four mutant strains with enhanced tyrosine conversion ability were obtained, among which the tyrosine conversion rate of strain number R1-14 reached 33.0% under 8 g/L salinity.By microbial pass-on assay, B.subtilis R1-14 strain showed good genetic stability.Through quantitative real-time PCR (qRT-PCR) analysis, the conversion of tyrosine by B.subtilis R1-14 was achieved through both aspartate aminotransferase (AST) and polyphenol oxidase (PPO), which AST enzyme played more important role.Finally, B.subtilis R1-14 strain was applied in broad bean paste fermentation.Results showed that the application of mutant strain R1-14 could significantly reduce the tyrosine concentration in mash by 45.7%, which was better than starting strain.The above results showed that the mutant B.subtilis R1-14 strain had a good tyrosine conversion rate and could significantly reduce the tyrosine content in broad bean paste, which had potential application value for preventing the occurrence of white spots.

Key words： tyrosine; Bacillus subtilis; atmospheric and room temperature plasma; broad bean paste; white spot substance

Cite this article

GU Nianwen , CHEN Huating , LIU Chunfeng , NIU Chengtuo , LI Qi . Screening of microbial strains with strong tyrosine conversion ability and its application in broad bean paste[J]. Food and Fermentation Industries, 2025 , 51(20) : 53 -60 . DOI: 10.13995/j.cnki.11-1802/ts.042259

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