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食品与发酵工业  2021, Vol. 47 Issue (3): 181-187    DOI: 10.13995/j.cnki.11-1802/ts.024783
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
七种竹叶品种挥发性化合物的统计学评价
韦杰1, 尹雅洁1,2,3, 江伟2*, 宋层业1, 魏灵1, 廖志力1, 韩兴林2
1(四川省宜宾竹海酒业有限公司,四川 宜宾,644304)
2(中国食品发酵工业研究院,国家酒类品质与安全国际联合研究中心,北京,100015)
3(湖北师范大学 生命科学学院,食用野生植物保育与利用湖北省重点实验室,湖北 黄石,435002)
Study on statistical evaluation techniques of volatile substances from seven kinds of bamboo leaves
WEI Jie1, YIN Yajie1,2,3, JIANG Wei2*, SONG Cengye1, WEI Ling1, LIAO Zhili1, HAN Xinglin2
1(Sichuansheng Yibin Zhu Hai Jiu Ye Co.Ltd, Yibin 644304, China)
2(International Joint Research Center of Quality and Safety of Alcoholic Beverages, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China)
3(Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China)
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摘要 竹叶及竹叶提取物在白酒、黄酒、保健饮料中应用广泛,基于顶空固相微萃取-气相色谱-质谱联用(headspace-solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)和统计学分析手段研究7种竹叶中的挥发性物。根据谱库检索和保留指数对比鉴定出7种品种竹叶中142种挥发性物质,包括了酯类、醇类、芳香类、醛类、酮类、杂环类、酸类、醚类、萜类共9大类物质,其中己醛、顺-2-己烯醛、甲基庚烯酮等10种物质为7种竹叶共有挥发性成分。对7种竹叶化合物分类统计发现,醛类物质以顺-2-己烯醛为主,相对含量范围在26.14%~62.26%;酯及内酯类物质中癸酸甲酯相对含量较高;酮类物质相对含量较高的为α-紫罗酮;醇类物质以苯乙醇为主;萜类物质是竹叶的主要成分,芳樟醇的相对含量最高。主成分分析得到3个主成分的贡献率为73.52%,其中酯及内酯类物质和芳香类物质在PC1上距离较近,对PC1正向贡献较大。采用聚类分析发现黄竹叶和其他6个品种竹叶挥发性差异较大,箭、苦、斑竹叶挥发性相似,麻、绵、慈竹叶挥发性差异不大。
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韦杰
尹雅洁
江伟
宋层业
魏灵
廖志力
韩兴林
关键词:  竹叶  顶空固相微萃取-气相色谱-质谱法(HS-SPME-GC-MS)  挥发性物质  主成分分析  聚类分析    
Abstract: Bamboo leaves and bamboo leaf extracts were widely used in Chinese baijiu, Chinese yellow rice wine and health drinks. The volatile substances of seven kinds of bamboo leaves were studied based on head space solid phase microextraction (HS-SPME), gas chromatography mass spectrometry (GC-MS) and statistical analysis in order to support theoretical foundation for the flavor of bamboo liquor. One hundred and forty two volatile substances were accurately identified from seven kinds of bamboo leaves by the index retrieval and retention index validation which including esters, alcohols, aromatic compounds, aldehydes, ketones, heterocycles, acids, ethers and terpenes. And among which nine substances such as hexanal, 2-hexenal and methyl heptene were common volatile components in seven kinds of bamboo leaves. According to the classified statistics of seven kinds of bamboo leaf compounds, it was found that cis-2-hexenal was the main aldehydes and the relative content range from 26.14% to 62.26%. The content of methyl decanoate (23.84%-49.96%) in esters and lactones were relatively higher, and the content of α-ionone in ketones was higher which between 0% and 53.33%. The phenyl ethanol was the main compound of alcohols, ranged from 18.49% to 75.65%. Moreover, terpenoids were the main components of bamboo leaves, and the relative content of linalool (39.12%-100%) was the highest. Three principal components were extracted by PCA (the contribution rate was 73.52%), in which esters and lactones and aromatic substances were closer on the PC1 and made a greater positive contribution to PC1. The cluster analysis showed that Dendrocalamus membranceus Munro were quite different from with the other six varieties, and the flavors of Fargesia spathacea Franch, Pleioblastus amarus and Phyllostachys bambusoides f. lacrima-deae bamboo leaves were similar, meanwhile, endrocalamus latiflorus Munro, Lingnania intermedia and Pleioblastus amarus leaves had little difference.
Key words:  bamboo leaves    head space-solid phase microextraction-gas chromatography-mass spectrometry    volatile compounds    principal component analysis    cluster analysis
收稿日期:  2020-06-17      修回日期:  2020-08-19           出版日期:  2021-02-15      发布日期:  2021-03-08      期的出版日期:  2021-02-15
基金资助: 国家自然科学基金项目(51965009)
作者简介:  韦杰国家级白酒评委和尹雅洁硕士研究生为共同第一作者(江伟教授为通讯作者,E-mail:jiangweigyig@163.com)
引用本文:    
韦杰,尹雅洁,江伟,等. 七种竹叶品种挥发性化合物的统计学评价[J]. 食品与发酵工业, 2021, 47(3): 181-187.
WEI Jie,YIN Yajie,JIANG Wei,et al. Study on statistical evaluation techniques of volatile substances from seven kinds of bamboo leaves[J]. Food and Fermentation Industries, 2021, 47(3): 181-187.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.024783  或          http://sf1970.cnif.cn/CN/Y2021/V47/I3/181
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