Abstract: High throughput sequencing was applied to analyze the microbial community structure of Daqu and its production environment. Microbial source tracking was further applied to analyze the origins of microbiota in new Daqu. The results showed that microorganisms in environmental samples from outdoor to indoor were gradually selected and enriched. From new Daqu to mature Daqu, the diversity of bacterial communities increased, while the diversity of fungal communities decreased. The main bacteria in the new Daqu were Weissella, while the bacteria in the mature Daqu were mainly Lactobacillus. Aspergillus and Rhizopus were the predominant fungi in both the new Daqu and the mature Daqu. Raw materials (89.3%) were the main contributor of bacterial in new Daqu, followed by straw mats (5.6%). Outdoor ground (55.18%) was the main contributor for fungi in new Daqu, followed by indoor roof (23%). This study analyzed the source of the medium-high temperature Daqu microbiota and provided a theoretical basis for optimizing the Daqu making process.
周天慈,何宏魁,周庆伍,等. 基于高通量扩增子测序技术解析中高温大曲微生物来源[J]. 食品与发酵工业, 2021, 47(16): 66-71.
ZHOU Tianci,HE Hongkui,ZHOU Qingwu,et al. Exploring the source of microbiota in medium-high temperature Daqu based on high-throughput amplicon sequencing[J]. Food and Fermentation Industries, 2021, 47(16): 66-71.
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2021, Vol. 47 
