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Food and Fermentation Industries    2022, Vol. 48 Issue (20) : 260-264     DOI: 10.13995/j.cnki.11-1802/ts.031216
Rapid determination of chemical components in fruit plum based on ultra performance liquid chromatography-tandem mass spectrometry technique
XIA Mengqiu1,2, WANG Xingchen1,2, YANG Lanxiang1,2, YUE Jingyi1,2, WANG Rui1,2, CAO Kan1,2, CHENG Wangkai1,2*
1(Wuhu Institute of Technology, Wuhu 241000, China)
2(Wuhu Life and Health Engineering and Technology Research Center, Wuhu 241000, China)
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Abstract  ultra performance liquid chromatography-tandem mass spectrometry was used to determine the chemical components in fruit plum. The analysis conditions were as follows. The chromatographic separation was performed using a ZORBAX SB-C18 (3.0 mm×100 mm, 3.5 μm) column, and the temperature of the column was maintained at 35 ℃. The mobile phases were 0.2% formic acid (A) and acetonitrile (including 0.2% formic acid) (B), respectively, using a binary gradient elution at the flow rate of 0.2 mL/min. Results showed that sixteen chemical components were successfully determined in fruit plum, including 10 organic acids, 4 flavonoids, 5-hydroxymethylfurfural, and bitter amygdidine. The main chemical compositions of fruit plum were successfully and quickly determined in this paper and the results could provide the theoretical basis for further study of the efficacy basis and mechanism of action.
Keywords fruit plum      ultra performance liquid chromatography-tandem mass spectrometry      chemical composition     
Issue Date: 18 November 2022
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