Preparation of mercury ion aptamer affinity column and  its application in aquatic products

LU Minghui; ZHENG Wenxiu; WU Shijia; WANG Zhouping; DUAN Nuo

doi:10.13995/j.cnki.11-1802/ts.040472

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Food and Fermentation Industries ›› 2025, Vol. 51 ›› Issue (5) : 197-202. DOI: 10.13995/j.cnki.11-1802/ts.040472

Preparation of mercury ion aptamer affinity column and its application in aquatic products

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Abstract

Heavy metal pollution has become a major agriculturally environmental concern in China.Because of its wide range of pollution sources, long toxic residues, biological enrichment and difficult to degrade, it poses a great threat to the agricultural ecological environment, food security, and human health.In this work, based on the specific binding of biotin and streptavidin, the mercury ion (Hg²⁺) specific aptamer was modified on the agarose gel to fill the column to make a mercury ion aptamer affinity column.The efficient removal of mercury ions was achieved through high affinity and specificity of aptamer to the target.The maximum column capacity of this aptamer affinity column was 0.025 mg and showed good specificity to mercury ions, and could be reused at least 12 times.The column was further applied to the analysis of Hg²⁺in fish sauce samples with good removal and no obvious effects on its nutritional components.This method s achieves stable and efficient removal, and has the advantages of low cost and good development prospects.

Key words

aptamer / affinity column / heavy metals hazards / Hg²⁺ / aquatic products

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LU Minghui, ZHENG Wenxiu, WU Shijia, WANG Zhouping, DUAN Nuo. Preparation of mercury ion aptamer affinity column and its application in aquatic products[J]. Food and Fermentation Industries, 2025, 51(5): 197-202 https://doi.org/10.13995/j.cnki.11-1802/ts.040472

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