The protective effect of wheat oligopeptides on oxidative stress in intestinal epithelial cells in vitro

ZHANG Ya-zhuo,JIANG Si-meng,WEI Ying,MA Yong,WANG Xiang-hong,GU Rui-zeng,CAO Ke-lu

Food and Fermentation Industries ›› 2015, Vol. 41 ›› Issue (1) : 46.

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PDF(369 KB)
Food and Fermentation Industries ›› 2015, Vol. 41 ›› Issue (1) : 46.

The protective effect of wheat oligopeptides on oxidative stress in intestinal epithelial cells in vitro

  • ZHANG Ya-zhuo,JIANG Si-meng,WEI Ying,MA Yong,WANG Xiang-hong,GU Rui-zeng,CAO Ke-lu
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Abstract

The purpose of this experiment was to study the protective effect of wheat oligopeptides on oxidative stress in intestinal epithelial cells in vitro.The experiment was divided into five groups,namely control group Ⅰ (blank group including 0 μmol/L H2O2),control group Ⅱ (oxidative stress group including 100 μmol/L H2O2),treatmentgroup Ⅰ,treatment group Ⅱ,and treatment group Ⅲ (adding 1,5 and 10 mg/mL wheat oligopeptides under the condition of controlled Ⅱ,respectively).MTT method was used to compare the proliferation rate of oxidative stress damaged Caco-2 cell after treated by wheat oligopeptides for different incubation time (12,24 and 48 h).The changes of the LDH,SOD and MDA contents were investigated using the supernate and lysis solution of Caco-2 cell after different incubation time (12,24 and 48 h).Results showed that wheat oligopeptides had a significant protective effect,reduced the cytotoxicity and increased the cell proliferation rate at the concentration of 1 mg/mL.The SOD and MDA contentof the treatment group was significant different from the oxidative stress group at the same concentration.The protective effect increased with the extension of incubation time.Compared with the group incubated for 12 h,the protective effects were significant different in the groups incubated for 24 and 48 h.It was concluded that wheat oligopeptides had certain protective effect on oxidative stress damagedintestinal epithelial cells,and the protective effectwas positive correlated withthe concentration and time within a certain scope.

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ZHANG Ya-zhuo,JIANG Si-meng,WEI Ying,MA Yong,WANG Xiang-hong,GU Rui-zeng,CAO Ke-lu. The protective effect of wheat oligopeptides on oxidative stress in intestinal epithelial cells in vitro[J]. Food and Fermentation Industries, 2015, 41(1): 46
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