The bacterial retention performances of mixed cellulose membrane,polycarbonate membrane,cellulose nitrate membranes and cellulose acetate membranes with 13 mm-diameter were investigated under the same conditions.The total plate count of bacteria in the tested sample was 10 CFU/mL.The filtrate volume was 50 mL,and the filtration was repeated 5 times.When the aperture was 0.22 μm,the average retention rates of four membranes were 100％,94％,84％ and 72％,respectively.When the aperture was 0.45 μm,they were 92％,82％,70％ and 70％,respectively.Therefore,the best membrane to retain bacteria was mixed cellulose membrane with 0.22 μm aperture.50 mL samples respectively containing bacteria 42 CFU/mL,20 CFU/mL,10 CFU/mL and 4 CFU/mL were separately filtered through mixed cellulose membrane.The bacteria on the membranes were determined by ATP bioluminescent method.There was a good linear correlation between fluorescent value and the bacteria concentration(R2 ＞ 0.9).The experiment results proved that mixed cellulose membrane with 0.22 μm aperture could meet the requirements about concentration and measurement of low-level bacteria content sample by ATP bioluminescent method.