Study on capillary electrophoresis fingerprints of alkaloids from lentinus edodes

HE Jin-zhe,MAO Yi-jie,FENG Ting-ting,SUN Pei-long

Food and Fermentation Industries ›› 2017, Vol. 43 ›› Issue (3) : 212.

PDF(744 KB)
PDF(744 KB)
Food and Fermentation Industries ›› 2017, Vol. 43 ›› Issue (3) : 212.

Study on capillary electrophoresis fingerprints of alkaloids from lentinus edodes

  • HE Jin-zhe,MAO Yi-jie,FENG Ting-ting,SUN Pei-long
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Abstract

Accelerated solvent extraction was used in extracting alkaloids from lentinus edodes and its analysis was performed by capillary electrophoresis.The optimized conditions of capillary electrophoresis were:background electrolyte 25 mmol/L borax-10 mmol/L β3-cyclodextrin;pH 9.5;the running voltage 20 kV;the sample size 20 psi × 5 s;the column temperature 25 ℃ and the detection wavelength 214 nm.Capillary electrophoresis methodology analysis showed that the method is precision,stable and reproductive.They were meet with the requirements of fingerprints.The capillary electrophoresis fingerprints of the alkaloids of letinous edodes in different regions were established.We found out 14 common peaks from the fingerprints,and evaluated the similarity of 10 batches of lentinus edodes through professional similarity evaluation system.The results showed that similarity of majority of those lentinus edodes were above 0.8 which indicate that the method can be served as a novel reference to identify and control the quality of lentinus edodes.

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HE Jin-zhe,MAO Yi-jie,FENG Ting-ting,SUN Pei-long. Study on capillary electrophoresis fingerprints of alkaloids from lentinus edodes[J]. Food and Fermentation Industries, 2017, 43(3): 212
PDF(744 KB)

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