Conjugated fatty acids refers to a group of positional and geometric isomers of polyunsaturated fatty acids,including conjugated linoleic acid (CLA),conjugated linolenic acid (CLNA) and conjugated stearidonic acid (CSA).CLA and CLNA were derived from linoleic acid and linolenic acid,respectively,with a variety of healthy-associated functions,such as anti-cancer,anti-atherosclerotic,immunity improvement and anti-obese,which have become a hot research topic in functional lipids.In this paper,twenty-two of Lactobacillus plantarum isolated from Paocai were analyzed for conjugated fatty acids production via GC-MS.The results indicated that CCFM47 and CCFM232 had high conversion capability of both CLA and CLNA.Further analysis showed that the two strain convert over 30％ linoleic acid to CLA,total CLA in the supernatant fluid reached 0.1664 mg/mL and 0.1514 mg/mL.Additionally,the two strains could convert over 60％ of α-linolenic acid to CLNA,with total CLNA 0.1964 mg/mL and 0.1721 mg/mL in the supernatant fluid,respectively.