A method was developed for the simultaneous determination of 7 synthetic phenolic antioxidants (SPAs),including gallic acid (GA),propyl gallate (PG),tertiary butylhydroquinone (TBHQ),isoamyl gallate (IAG),octyl gallate(OG),dodecyl gallate (DG) and butylated hydroxytoluene (BHT),in yeast products by using high performance liquid chromatographic (HPLC) method.The yeast products was extracted with acetonitrile and then cleaned up by PCX.The separation of analytes was performed on a JADE-PAK C18 column (4.6 mm × 150 mm,5 μm) by gradient elution using 1％ aqueous acetic acid solution and methanol as mobile phase.The sample was separated and detected with photodiode array detector (DAD).The qualification analysis was performed by using retention time and UV spectrum,and the quantification analysis was based on the detection wavelength of 280 nm.The calibration curves showed good linearity for seven synthetic phenolic antioxidants (SPAs),and the correlation coefficients (r2) were higher than 0.994.The limits of quantification (LOQs,S/N ＞ 10) were in the range of 1-10 mg/kg.The average recoveries were in the range of 79.7 ％-102.4 ％ and 79.3 ％-107.4 ％ for seven antioxidants respectively in active dry yeast and yeast extract,with the relative standard deviations (RSDs) of 2.5 ％-8.7 ％ and 2.7 ％ -9.6 ％ at the spiked levels of 1,2,10 LOQ.The method is accurate,rapid and sensitive,and suitable for monitoring of seven synthetic phenolic antioxidants (SPAs) in yeast products.