Enzymatic properties of recombinant Athrobacter ramosus S34 MTSase and MTHase and optimization of application conditions for production of trehalose

WANG Kui,SU Ling-qia,WU Jing,CHEN Sheng

Food and Fermentation Industries ›› 2017, Vol. 43 ›› Issue (7) : 1.

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Food and Fermentation Industries ›› 2017, Vol. 43 ›› Issue (7) : 1.

Enzymatic properties of recombinant Athrobacter ramosus S34 MTSase and MTHase and optimization of application conditions for production of trehalose

  • WANG Kui,SU Ling-qia,WU Jing,CHEN Sheng
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Abstract

The production of trehalose from starch is an effective method under the combined action of maltosyltrehalose synthase (MTSase) and maltosyltrehalose hydrolase (MTHase).The optimum temperature of MTSase was 45℃,the optimal pH was 7.0,and the optimal temperature of MTHase was 55℃,the optimum pH was 6.0.Then trehalose was produced by the two enzymes together,and the reaction conditions were optimized.The effects of reaction temperature,initial pH,substrate DE value,enzymes amounts and substrate concentration on trehalose yield were investigated.The optimal conditions were as follows:the reaction temperature was 45 ℃,the initial pH was 8.5,the substrate DE value was 8.5 and the amount of MTSase and MTHase was 15.75 U/g and 7.5 U/g,respectively.

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WANG Kui,SU Ling-qia,WU Jing,CHEN Sheng. Enzymatic properties of recombinant Athrobacter ramosus S34 MTSase and MTHase and optimization of application conditions for production of trehalose[J]. Food and Fermentation Industries, 2017, 43(7): 1
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