Research herein was focused on the best condition for preparation of Lactobacillus plantarum agent with soybean meal containing 2％ glucose.The number of viable cells after drying was used as an indicator to investigate the production process.The production process was optimized through one-factor experiment combined with response surface methodology.After fermentation,content of crude protein,acid soluble protein,free amino acids,trypsin inhibitor,urease,and phytic acid,as well as protein digestibility were determined.Results showed that Lactobacillus plantarum achieved the highest biomass after incubation at 30.6 ℃ for 46.40 h,with substrate to water ratio of 1∶0.61 and inoculum concentration of 4.45％.The content of crude protein,acid soluble protein,and total free amino acids,and protein digestibility were increased 4.55％,1.24％,50.47 mg/g and 4.21％ respectively.The content of trypsin inhibitor,urease,and phytic acid were decreased 94.59％,91.64％,and 30.22％,respectively.The number of Lactobacillus plantarum could reach 9.88 lg(CFU/g) under the best drying condition of 50℃ hot air for4 h.