Three kinds of swim bladders from octopus, large yellow croaker, mackerel in East China Sea were studied to analyze the amino acids, fatty acid components and mineral elements of the swim bladders by GC-MS, ICP-MS and other technologies. The results showed that the indexes of the swim bladder of the large yellow croaker were better than those of octopus and mackerel. The amino acid scoring criteria showed that the concentration of flavor amino acid in the large yellow croaker swim bladder is 58.55 g/100g.Gly, Ala, Arg and other functional amino acid content is high,meaning the swim bladder of the large yellow croaker had a very good application prospect on the production of seafood seasonings. The main fatty acids of swim bladder of the large yellow croaker were monounsaturated fatty acids and polyunsaturated fatty acids, respectively occupying 13.09% and 55.13%.Compared with those in the octopus and mackerel, the contents of K, Na, Ca, Fe and Zn in the bladder of the large yellow croaker were higher, and the contents of Fe and Ca were significantly higher, thus indicating the swim bladder of the large yellow croaker was the good food source of supplementing Fe and Ca; and the contents of Hg and As in the swim bladder of large yellow croaker did not exceed the statutory content of national standard, meaning it was a kind of safe food.