The effects of glucose on xylitol fermentation byCandida tropicalis SFX-Y9 was studied in this paper. Results showed that strains SFX - Y9 with glucose as carbon source to cultivate seeds can shorten training time, improve the concentration of cells, and had little influence upon conversion of xylose.When the fermentation medium was supplemented with a small amount of glucose, it can increase the consumption rate of xylose and shorten the fermentation cycle. Through the control of dissolved oxygen and the glucose fed-batchcan further increase the rate of xylose consumption in 5L fermentor, and the conversion rate of xylose increased by about 15%.Under the condition ofoptimal oxygensupply and glucose as a secondarycarbon source, the production rate of xylitol can reach 5.0 g/L/h,and the rate of conversion can reach more than 80%.The production cost of xylitol by microbial conversion can compete with the chemical hydrogenation, and it has a bright application future.