In this paper, sugarcane bagasse residue, polypropylene microfiber membrane and cotton cloth were adopted as supports to immobilizeActinobacillussuccinogenesfor the fermentative production of succinic acid. The usage of supports was optimized to improve the yield of succinic acid and repeated batch fermentation was conducted to study the reusable stability of supports. Polypropylene microfiber membrane performed best in the production of succinic acid while cotton cloth performed best in the reusable stability test. As a result, cotton cloth was used in the construction of a fibrous-bed bioreactor. In the study of the effect of pH regulator on bioreactor efficiency, there were 32% and 20% increase in succinic acid concentration when using MgCO3as the pH regulator compared with that using NH3×H2O-Na2CO3and KOH-K2CO3. Repeated fed-batch fermentation was operated in the bioreactor with the average batch time of 35 h and the total operation time of 175 h. Results showed that the average concentration, productivity and yield of succinic acid were87.8 g/L, 2.51 g/L×h and 0.86 g/g, respectively. Results showed that the fibrous-bed bioreactor reported in this paper helped to achieve high concentration, high productivity and high yield of succinic acid.