In this paper, sugarcane bagasse residue, polypropylene microfiber
membrane and cotton cloth were adopted as supports to immobilizeActinobacillussuccinogenesfor the
fermentative production of succinic acid. The usage of supports was optimized
to improve the yield of succinic acid and repeated batch fermentation was
conducted to study the reusable stability of supports. Polypropylene microfiber
membrane performed best in the production of succinic acid while cotton cloth
performed best in the reusable stability test. As a result, cotton cloth was
used in the construction of a fibrous-bed bioreactor. In the study of the
effect of pH regulator on bioreactor efficiency, there were 32% and 20%
increase in succinic acid concentration when using MgCO3as the pH
regulator compared with that using NH3×H2O-Na2CO3and KOH-K2CO3.
Repeated fed-batch fermentation was operated in the bioreactor with the average
batch time of 35 h and the total operation time of 175 h. Results showed that
the average concentration, productivity and yield of succinic acid were87.8
g/L, 2.51 g/L×h and 0.86 g/g,
respectively. Results showed that the fibrous-bed bioreactor reported in this
paper helped to achieve high concentration, high productivity and high yield of
succinic acid.
CHEN Peng-cheng et al
. ActinobacillussuccinogenesImmobilization and Using Fibrous-bed Bioreactor
for the Production of Succinic Acid[J]. Food and Fermentation Industries, 2017
, 43(12)
: 7
-12
.
DOI: 10.13995/j.cnki.11-1802/ts.015305