Monascusspp. is a traditional Chinese medicinal and edible microbial resource. Its main metabolites includingMonascuspigment (MPs), monacolin K (MK), etc. The previous results indicated that glycerol could improve MK and MPs production byMonascus. AMonascusstrain,Monascus pilosusMS-1, which could produce high yields of MK and MPs but non citrinin was used. And the effects of crude glycerol and analytical grade glycerol on the growth and production of MK and MPs by MS-1 were carried out. The results showed that analytical grade glycerol could improve MK and MPs production in solid-state fermentation by MS-1. The most suitable analytical grade glycerol concertations for MK and MPs produciton in solid-state fermentation was 6% and 3%, respectively, and the yields of MK and MPs was 1.57 and 1.53 folds to that of control, respectively. The highest yields of MK and MPs were obtained when 6% crude glycerol from fatty alcohol with 90% purity was used and the homologues were also more than that of control and which adding analytical grade glycerol in solid-state fermentation (p<0.05). In liquid fermentation, pH values of fermentation broths and biomass were significantly decreased and increased, respectively, when crude glycerol and analytical grade glycerol with concentration of 40 g/L were used (p<0.05). MK and MPs production were significantly enhanced when crude glycerol and analytical grade glycerol were used (p<0.05). MK and MPs yields were 11.57 and 11.08 folds to that of control when crude glycerol from biodiesel with 90% purity and crude glycerol from fatty alcohol with 90% purity were used, respectively. Moreover, the species of MK and MPs were increased compared to that of control. Crude glycerol possess huge potential use for MK or MPs production.