Effects and conditions of stable chlorine dioxide combined with heat treatment were explored to prolong the storage time of Chestnut. With preservation rate as index, a response surface methodology was used to investigate the effects ofChlorine Dioxide mass concentration, heat treatment temperature and time on the preservation rate to optimize the storage conditions.The results showed that synergy effects existed between Chlorine Dioxide mass concentration and treatment temperature and between Chlorine Dioxide mass concentration and treatment time. The best storage conditions wereChlorine Dioxide mass concentration 167 mg/L ,treatment temperature 57 ℃ , treatment time 22 min. Under the conditions，after storage at temperatures of 5-15 ℃ for 60 days, the preservation rate of Chestnut was up to 93.61%。