A new method based on ultra performance convergence chromatography (UPC2) was developed for analysis of free amino acids in fruit wine. They were separated on an Acquity UPC2Torus Diol (3.0 ×100mm, 1.7 μm) with a mobile phase consisted of supercritical CO2and methanol: acetonitrile (1: 1 v/v, with 2.5 mmol/L ammonium acetate as mobile phase modifier), the flow rate is 2.0 mL/min and DAD detection was set at 280 nm with a compensation range of 330-430 nm, back pressure is 1800 psi. The results showed good linearity (R2= 0.9990-0.9998) and high resolution higher than 1.22. The limits of detection (LOD) and quantification (LOQ) ranged from 23-57 ng/L and 70-178 ng/L, respectively. The linear range was between 20 and 400 μg/mL. The spiked recoveries were ranged from 85.6% to 101.12%. It was applied for the free amino acids analysis of selected pitaya wine, which could also provide a new method for the analysis of free amino acid distribution in other food matrix from different sources.