Using the national standards methods, the appearance, grain weight, physical-chemical properties, nutrition components and bio-active compounds of five kinds of red raspberry (Heritage, FertodZamatos, Autumn Bliss, U.S. Raspberry and European red) seeds were analyzed and compared. The results showed that red raspberry seeds were small grain seeds with thousand seed weight of 1.06-1.60 g, water content of 3.23%-8.78%, crude fat content of 12.69%-16.65%, crude protein content of4.64%-8.54%, total sugar content of2.00%-4.26%, reducing sugar contentof 0.97%-2.40%,crude ash contentof0.84%-1.57%,total polyphenols contentof 15.21-18.53 mg/g, flavonoids contentof 8.19-11.50 mg/g, procyanidinscontentof 19.43-31.10mg/g.The contents of vitamin E in different varieties of red raspberry seed were 33.80-52.59 μg/g, but vitamin A, D, K were not detected.Proximate analysis showed that the raspberry seeds were rich in mineral elements, proved to be a good source for minerals Ca,Co,Fe,K,P, and Autumn Bliss seeds had the most abundant mineral element content which wasup to 3520.51 μg/g.The scientific and theoretical basis were provided for red raspberry seeds fully utilized.