Staphylococcus aureus detection by reverse transcription loop-mediated isothermal amplifi cation#br#

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  • (China National Research Institute Food & Fermentation Industries, Beijing 100015)

Online published: 2018-02-05

Abstract

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an amplification reaction using RNA as a template for detection. A real-time fluorescent RT-LAMP method for the detection ofStaphylococcus aureuswas established by optimizing primers designed for specific conserved nuc gene and optimizing the reaction conditions. The strain-specific and the sensitivity of pure culture ofStaphylococcus aureusand artificial contamination of skim milk samples were detected by this method. The RT-LAMP amplification reaction was completed at 65°C for 30minutes. The method had a strong strain specificity, the detection limit of pureStaphylococcus aureuswas 20 fg/μL and the sensitivity was 230 CFU/g in the detection of artificial contaminated skim milk samples. The established real-time fluorescent RT-LAMP method can provide a powerful means for rapid detection ofStaphylococcus aureusin the field.

Cite this article

LIANG Yu-lin et al . Staphylococcus aureus detection by reverse transcription loop-mediated isothermal amplifi cation#br#[J]. Food and Fermentation Industries, 2018 , 44(1) : 217 . DOI: 10.13995/j.cnki.11-1802/ts.015664

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