A silica gel solid phase of the neutral alumina extraction-high performance liquid chromatographic method was established for the analysis of Tert-Butylhydroquinone(Tert-Butylhydroquinone,TBHQ )in the Vegetable oil. the amount of silica gel,the amount of oil,the polarity of the eluent,the velocity and the specifications of chromatography column were optimized in this experiment. Add recovery rate and precision test experiments showed that: the recovery rate of 3.1?μg、5.4?μg and 13.6?μg were 95.6%-103.7%, RSD % were 3.5%-5.1%,The results of TBHQ content in the same sample were basically the same as that of NY/T1602-2008,which showed  that the method has good accuracy reliability and repeatability,Could be used for the determination of TBHQ content in vegetable oil.