Encapsulated enzymes by using yeast spores is a new enzyme-immobilized technology. It was reported that the activity of encapsulated enzymes was largely improved bySaccharomyces cerevisiaespores ofosw2Δwhich has mild spore wall defective and theosw2Δspore-encapsulate enzymes also exhibit resistance to environmental stresses and have a good reusability. To investigate whetherkre1Δspores have similar ability, the creatininase gene was transformed into yeast cells and the properties of encapsulated enzymes were examined. The results suggested the activity of the creatininase encapsulated inkre1Δspores was similar toosw2Δspores but was significantly higher than that of wild type spores. Compared with free enzymes, likeosw2Δ,encapsulated creatininase inkre1Δspores exhibits resistance to environmental stresses and has a good reusability. The spore-encapsulated creatininase inkre1Δandosw2Δshows highest activity at 60oC, pH 6.0. Moreover, thekre1Δspore-encapsulated enzymes are resistant to high temperature and exhibit a wide range of pH tolerance as same as that ofosw2Δspores. Therefore,kre1Δspores can be used as a superior carrier of enzyme capsules.