In yeast cell, glutathione (GSH) cannot be automatically
discharged from the cell, and so the overexpression of GSH is hard to achieve.
The effects of cinnamaldehyde and citral on synthesis of GSH were investigated.
The results showed that the growth of yeast cells were inhibited by cinnamon aldehyde
and citral, but the secretion of GSH were inhanced in certain concentrations. The
treated yeast cells were used to catalize the synthesis of GSH, and the yield
of GSH was increased by 59.3% and 46.3% after the cells were treated by 45 μg/mL
cinnamon aldehyde or 100 μg/mL citral. The yeast cells were cultured under the
following condition: glucose 45 g/L, medium volume 70 mL/250 mL, 24 h shaking and
12 h static culture, 25 g/L glucose was added after shaking, cinnamon aldehyde
and citral were added to control the cell growth. After the treatment of 45 μg/mL
cinnamon aldehyde or 100 μg/mL citral, the total yield of GSH was 580 mg/L or
551 mg/L, 107% or 96.8% higher than the untreated group.
SUN Yi-ran et al
. Effect of yeast growth regulation by cinnamaldehyde or citral on glutathione synthesis[J]. Food and Fermentation Industries, 2018
, 44(2)
: 15
.
DOI: 10.13995/j.cnki.11-1802/ts.015775