Optimization and characterization of 2-phenylethanol bioconversion by thermotolerant yeast Kluyveromyces marxianus

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  • 1(College of food and bioengineering, Henan University of Science and Technology, Luoyang 471023, Henan, China) 2(Luoyang Engineering and Technology Research Center of Microbial Fermentation, Luoyang 471023, Henan, China)

Online published: 2018-03-14

Supported by

国家自然科学基金(31500052)

Abstract

The medium for the production of 2-phenylethanol (2-PE) by Kluyveromyces marxianusstrain LY1 were studied with the single factor and response surface methodology. The optimized medium contains 8.39?g/L L-phenylalanine, 43.25?g/L saccharose, 6.68?g/L yeast extract. The yield of 2-PE reached up to 1.450?g/L, increased by 51.0% compared with original condition. Then strain LY1 was further analyzed for its tolerance properties and high temperatural fermentation ability. The results showed that the highest tolerance to 2-PE of strain LY1 was 2.0?g/L at 36?℃ and 1.5?g/L at 42?℃. Moreover, the yield of 2-PE reached to 0.809?g/L at 42?℃. It would provide reference for production of 2-PE by thermotolerant yeasts at high temperature.

Cite this article

NIU Ming-fu et al . Optimization and characterization of 2-phenylethanol bioconversion by thermotolerant yeast Kluyveromyces marxianus[J]. Food and Fermentation Industries, 2018 , 44(2) : 15 . DOI: 10.13995/j.cnki.11-1802/ts.015833

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