The aim of this study was to screen the fine
fermentation strains of wheat distiller's grains and optimize its solid-state
fermentation technology to improve its feed quality. The content of acid
soluble protein in the fermentation product was the main factor to evaluate the
fermentation of wheat distiller's grains supplemented by wheat middings. The
optimal combination of strains that saved in the lab were studied by using a
single-factor test. And then optimize the fermentation substrate ratio and solid-state
fermentation conditions (inoculation amount, fermentation temperature and
fermentation time). On the basis of single factor experiment, response surface
methodology was used to optimize the solid-state fermentation conditions. The
optimum combination of strains was obtained by Lactobacillus R-02 and Bacillus
Subtilis KG109. The best fermentation substrates matrix proportion were as
follows: wheat distiller's grains was 38%, wheat middings was 62%. The optimum
solid-state fermentation conditions were as follows: inoculation amount was
1.22%, fermentation temperature was 33.45 ℃, fermentation time
was 6.78 days. Under these fermentation conditions, acid soluble protein was
4.33%. After optimization of the fermentation of wheat distiller's grains, feed
quality has been significantly improved.
LI Shan et al
. Screening of strains on fermentation and optimization of fermentation conditions in wheat distiller′ s grains used for
feed[J]. Food and Fermentation Industries, 2018
, 44(2)
: 105
.
DOI: 10.13995/j.cnki.11-1802/ts.015750