An extraction procedure and method of Rhodiola roseame polysaccharides (RP)and its antibacterial and antioxidant activity evaluation was established by response surface methodology(RSM) and BBD central componse design coupling aqueous two phase system was used to optimize the optimal extraction model parameter between extraction factors and yields of RP polysaccharide. The inhibition zone which used to estimate the antibacterial activities were determined by filter paper diffusion method and  agarophyte method. The scavenging effect to the hydroxyl radical and superoxide radical were detected with the oxidation of ferrous sulfate-salicylic acid- hydrogen peroxide and self-oxidation of pyrogallic acid system. The optimum extraction conditions were found to be extraction temperature 50℃, extraction time 37.5 min and ration of Liquid to materials 22.5 g/mL. Under these conditions, the extraction efficiency of RP polysaccharides achieve 16.64%. The result of antibacterial  activity indicatethat RP polysaccharides present extremely sensitive to staphyLococcus aureus with inhibition diameter of 21.1±0.09mm) and high Sensitivity tocolibacilluswith inhibition diameter of 16.4±0.02 mm, while present moderately sensitive to baciLLus subtiLiswith inhibition diameter of 12.6±0.04 mm.The rusult showed that RP polysaccharides possess a certain antimicrobial activity in vitro and have certain specific selectivity. The evaluation of antioxidant activity suggested that the RP polysaccharides exhibited significant antioxidant activity taking the vitaminC as the reference and obtain maximum scavenging activity of 65.1%(2.0 mg/mL) and 51.2%(2.5 mg/mL) respectively. The quadratic multinomial regression mode between extraction factors and yield was established and be used as the index of evaluating the qualitative and quantitative of extraction method by response surface methodology[1]andBBD design. The result showed that RP possess a certain antioxidant and antimicrobial activity in vitro and has application value for developing as natural antioxidant and antiseptic substance in food and pharmaceuticals industries, while to provide technical guidance and experimental data for Rhodiola roseame plant resources rational allocation and related products diversified application.