To investigate the effects of dual protein on exercise fatigue, 18 healthy and SPF grade wistar male rats were randomly divided into 3 groups: soy protein(n=6), whey protein(n=6), and dual protein groups(n=6). We used weight loaded force swimming test to evaluate anti-fatigue capacity. Tail venous blood was collected to detect the activity of creatine kinase and lactate dehydrogenase at certain time period. After six weeks of feeding, mean exhaustive time of the rats fed with dual protein can reach to135.8 s which was significantly higher than the whey protein group (88 s) and soybean protein group (99.8 s). Lactate dehydrogenase activity of dual protein group reached to pick value in 30 min and went back to the same level as others in 60 min. Thus, supplement of dual protein after exercise can help recover anti-fatigue quickly and improve the endurance capacity of wistar rats.