A novel detection method had been established to detect Cronobacter sakazakii by Loop-mediated isothermal amplification method coupled with an electric-free heater using calcium oxide and water to provide heat through the LAMP processing. Meanwhile, specific primers were designed based on ITS sequences of Cronobacter sakazakii for the rapid, efficient point-of-care testing on Cronobacter sakazakii of powered infant formula. Finally, the results were determined by agarose gel electrophoresis. Sensitivity of the above method was determined and this non-electric heating detection system was compared with the traditional electric heating method. At the same time, sensitivity experiments were performed at different ambient temperatures. The results showed that the sensitivities of Cronobacter sakazakii in the pure cultures were both 2.6×102 CFU/mL by using two types of technologies while those in artificial contaminated milk were both 4.2×102 CFU/g. This non-electric heating test system had good stability and the sensitivity of 102 CFU/g at environment temperature of 4-37 ℃.
