Vancomycin resistant genes of strain Enterococcus faecalis EC-12 were screened in this study. PCR specific for seven typical genes vanA, vanB, vanC1, vanC2/C3, vanD, vane and vanG were carried out. Results showed that all of them were negative in strain EC-12. This specific PCR method could be used to detect vancomycin-resistant Enterococcus sp.