The target geneALDH2 was synthesized according to the sequences of human acetaldehyde dehydrogenase 2 in GeneBank after codon optimization ofSaccharomyces cerevisiae. Then theALDH2 gene expression cassette TRP1L-URA3-TPIp-ALTH2-TPIt-TRP1R was constructed using fusion PCR and electro-transformed intoS.cerevisiaeW303-1A to produce the modified strain W303-ALDH2. The recombinant ALDH2 was obtained after purification by His TrapTMexcel affinity chromatography; and its molecular weight and activity was 56 kDa and 20.70 U/mg; respectively. The results showed that the optimal reaction pH value and temperature for recombinant ALDH2 were 5.0 and 35 ℃; respectively. Except Na+; the K+; Ca2+; Mg2+; Mn2+could increase the activity of ALDH2 in different degrees. When acetaldehyde was used as substrate; theKmvalue and maximum reaction rateVmaxof recombinant ALDH2 was 0.908 mmol/L and 114.94 U/mg; respectively. When NAD+was used as substrate; theKmvalue and maximum reaction rateVmaxof recombinant ALDH2 was 0.282 mmol/L and 58.82 U/mg; respectively.