Food and Fermentation Industries

Simultaneous determination of twenty-one sulfonamides residues in honey by QuEChERS ultra liquid chromatography-mass mass spectrometry

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  • (Zhongshan Subcenter of Inspection and Quarantine Technology Center; Guangdong Entry-Exit Inspection and Quarantine Bureau; Zhongshan 528403; China)

Online published: 2018-05-25

Abstract

The method for simultaneous determination of 21 sulfonamide residues in honey was developed by Ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Deuterium substituted standards were used as internal standards and added to the sample before extraction. The sample was dissolved by buffer solution (pH=4.00); extracted with 1% formic acid in acetonitrile; purified by salting-out and cleaned up by NH2 adsorbent. The mass spectrometer was operated in the positive ion mode using multiple-reaction monitoring for qualitative and quantization. The detection limit of the method was 0.12-2.46 μg/kg. The correlation coeficient of linear calibration curve was over 0.99 within the range of sulfonamides concentration 0.05-60 ng/mL. The average recoveries for 21 sulfonamide residues were 60.44%-107.80%. The method is simple、rapid、reliable、stable and sensitive. It's suitable for high throughout determination of 21 sulfonamide residues in honey.

Cite this article

RONG Yu-tang;ZHANG Xian-chen;ZHANG Peng-jie;LI Rong*; HUANG Wei-rong;CHEN Li-si . Simultaneous determination of twenty-one sulfonamides residues in honey by QuEChERS ultra liquid chromatography-mass mass spectrometry[J]. Food and Fermentation Industries, 2018 , 44(4) : 226 -233 . DOI: 10.13995/j.cnki.11-1802/ts.014783

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