Food and Fermentation Industries

Real-time fluorescence PCR in allergens detecting of wheat and hazelnuts

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  • (Chengdu Institute for Food and Drug Control; Chengdu 610045; China)

Online published: 2018-05-25

Abstract

Food allergies are in increasing issue in food safety. Therefore; the evaluation and detection of food allergens become more and more important. To quickly and accurately detect food allergens is an urgent need. At present; the most common method used in allergen detection are immunological detection; mass spectrometry and SPR technology. However; there are some limitations in these methods. We used molecular biology techniques and established a database of allergen genes for wheat and hazelnut; and designed primers by finding the specific sequences. In order to find a rapid; convenient and efficient method for the detection of allergen;we implemented a real time fluorescence PCR method for a rapid screening of the allergen gene. It is simple; low-cost; easy for operation and time saving. The detection limit was increased; and miss-dection rate was lowed. Through the applicability test and LOD experiments; we found the method is applicable for the identification of allergens; good repeatability and accuracy with the detecting limit of 1% .

Cite this article

SHANG Ke; ZHANG Biao; DUAN Qing-zi; ZHANG Yu; WANG Wei; LIANG Heng-xing* . Real-time fluorescence PCR in allergens detecting of wheat and hazelnuts[J]. Food and Fermentation Industries, 2018 , 44(4) : 234 -240 . DOI: 10.13995/j.cnki.11-1802/ts.014678

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