A ultimate performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method for the qualitative and quantitative determination of γ-hydroxybutyrate(GHB), γ-butyrolactone(GBL) and 1,4-butanediol(1,4-BD) in beverage was established. The samples were diluted by different multiples and filtered with 0.22 μm membrane. Three compounds were separated in 10 minutes on a column of Thermo Gold C18(150×2.1 mm,3.0 μm) with gradient elution using 2 mmol/L ammonium acetate(A) and acetonitrile(B) at 0.20 mL/min.Samples were monitored via multiple reaction monitoring (MRM) model.External standard curve was used for quantitative analysis. The method exhibited a linear range of 0.5-10.0 μg/mL(r>0.99) for all compounds and the limits of detection(LOD) was 0.5μg/mL. The recoveries of GHB, 1,4-BD and GBL were 81.1%-87.7%, 98.5%-99.8% and 107.5%-116.2%, respectively, and relative standard derivation(RSD) ranges were 3.3%-5.7%, 3.7%-5.3% and 3.5%-5.1%(n=6), respectively. The RSD of accuracy, repeatability, stability and recovery of the method were all less than 6%(n=6). The method was sensitive, simple and rapid, and can be applied to the detection of target substances in different beverages.
GONG Lei
,
HAN Zhi
,
LIU Jie
,
ZHU Xiao-ling
,
WANG Hui-xia
,
PENG Qing-zhi
. Simultaneous determination of γ-hydroxybutyrate and its precursor in beverages by ultimate performance liquid chromatography-mass spectrometry[J]. Food and Fermentation Industries, 2018
, 44(9)
: 262
-269
.
DOI: 10.13995/j.cnki.11-1802/ts.016215
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