The atrazine-degrading strains were screened using solid culture with high concentration of atrazine and high performance liquid chromatography (HPLC). The dynamics of growth and atrazine degradation rate were determined, and the effects of co-substrates and environmental condition on degradation rate were also evaluated. The results showed that 10 strains had degradative capability for atrazine with the degradation rate from 13.3% to 41.1%, and the maximum degradation rate was obtained with strain XQB-33. Among 10 strains, 4 strains (XQB-1, XQB-21, XQB-25, XQB-33) showed high atrazine degradation capacity with degradation rate of 38.1%, 34.6%, 37.3% and 41.1%, respectively．Furthermore, theses 4 strains reached logarithmic phase and showed the highest atrazine degradation rate after culture in liquid medium for 72 h. After addition of glucose, sodium citrate, sucrose and succinic acid sodium acetate as co-substrates, the degradation rate of 4 strains increased significantly, especially for addition of 0.5% glucose, the degradation rate of XQB-1 and XQB-1 increased by 15.2% and 14.3%, respectively. The environmental factors can affect the degradation efficiency. The optimum degradation conditions for these 4 strains were as follows: inoculation amount of 2.0%，initial atrazine concentration of 100-200 mg/L, initial pH value of 5-6, culture temperature of 30 ℃, and volume of 75-100 mL/150 mL.