Using 2.5 L bench-top fermenter, fermentation was optimized for succinic acid production of recombinantYarrowia lipolyticaPGC11505. The results showed that, the output of succinic acid was enhanced substantially after the optimization of rotation speed, aeration rates, initial concentrations of glycerol and peptone, pH value of the fermenter. For detail, production of succinic acid was doubled under the speed of 600 r/min compared with 400 r/min. Aeration rate of 1.0 vvm was appropriate compared with 0.5 vvm and 2.0 vvm. Oxygen was deficient when aeration rate was low and high aeration rate would waste power and energy . PGC11505 grew well and fermentation was done totally when the initial glycerol concentration was 100 g/L, and the output of succinic acid reached 21.0 g/L. 10.0 g/L peptone was optimal for succinic production. PGC11505 performed the best when the pH value was kept natural as compared with pH value of 4.0, 5.0 and 6.0, which achieved 26.3 g/L succinic acid and a yield of 0.27 g/g. Finally, fed-batch fermentation was carried out under natural pH value for 138 hours. A total of 192.5 g/L glycerol was consumed and 46.9 g/L succinic acid was produced with a yield of 0.24 g/g and productivity of 0.34 g/(g·h). After optimization, the output of succinic acid was improved by 4.7-fold.