Analysis on enzyme production of Schizophyllum commune fermented with cornbran fiber and cloning, expression and characterization of xylanase gene

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  • 1(Wuhan Polytechnic University, Biological and Pharmaceutical Engineering, Wuhan 430023, China) 2(Academy of State Administration of Grain, Beijing 100037, China)

Online published: 2018-07-04

Abstract

Corn bran is a by-product of corn starch processing.Most of it has not been fully utilized.Microbial fermentation and enzymolysis technology provide new ideas for the utilization of corn bran.Corn bran as the sole carbon source culture of Schizophyllum commune, hemicellulase activity in fermentation broth was determined and the results showed the highest xylanase activity was 7.45 U/mL, acetyl xylan esterase activity was 3.41 U/mL.α-L-arabinofuranosidase and α-glucuronidase activity was relatively low as 0.44 U/mL and 0.074 U/mL.The xylanase gene Xyn22 was cloned according to enzyme activity and its expression in E.coli BL21(DE3).Then purification and characterization of recombinant protein were completed.The optimum reaction temperature was 40 ℃, and the relative enzyme activity was high than 80% when the reaction temperature reached 45 ℃.The optimal pH was 5.0.The activity was about 80% in the range of 4.5- 6.5.Xyn22 was stable in the range of pH 3.0 to 10.5.Mg2+, Ba2+, EDTA and Hg2+ has significant inhibitory effect on the enzyme activity, Hg2+ could almost completely inactivated Xyn22.

Cite this article

WANG Jing-yu et al . Analysis on enzyme production of Schizophyllum commune fermented with cornbran fiber and cloning, expression and characterization of xylanase gene[J]. Food and Fermentation Industries, 2018 , 44(5) : 46 -51 . DOI: 10.13995/j.cnki.11-1802/ts.015592

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